List of Pre-approved Organisms and Chemicals
Preapproved Chemicals
The following chemicals are permitted in the BUGSS facility as long as they are in their original labeled container, specifying the identity, concentration, hazard rating and storage conditions. A MSDS must accompany the chemical and will be placed in the BUGSS MSDS file. For chemicals not on this white list specific written permission is required from the BUGSS safety officer (safety@bugssonline.org) before the chemical can be allowed into the facility.
- Tryptone
- Yeast Extract
- Peptone
- Amino Acids
- L-proline
- Vitamins
- Agar
- Glucose
- Sucrose
- Galactose
- Arabinose
- Raffinose
- Agarose
- Tris-HCL
- Tris Base
- Sodium acetate
- Sodium chloride
- Calcium Chloride
- Ammonium chloride
- Magnesium chloride
- Magnesium sulfate
- Gel-Red
- Brome phenol Blue
- Glycerol
- Triton X-100
- Tween 20
- IGEPAL®CA-630
- Ethanol (quantity limited)
- Isopropanol (quantity limited)
- DMSO (quantity limited)
- IPTG
- Ampicillin
- Kanamycin
- Chloramphenicol
- Tetracycline
- Lithium acetate
- Potassium Chloride
- Acetic Acid (quantity limited)
- HCL (quantity limited)
- NaOH (quantity limited)
- Lysozyme
- RNase A
- Restriction and other DNA modifying enzymes
- Zymolyase
- Calf Thymus DNA
- Anti-mouse IgG
- BCIP®/NBT solution
- EDTA
- Murashige & Skoog Basal medium
- Murashige & Skoog Basal salts with minimal organics
- Sodium dodecyl sulfate
- Tryptic soy broth
- D-mannitol
- 10% FeCl3
- Instant Ocean
- PEG-3350
- Sephadex G-50
- Lactose
- Potassium phosphate monobasic
- Potassium phosphate dibasic
- Yeast nitrogen base w/ ammonium sulfate w/o amino acids
- Simply Blue Safe Stain
- Tris-Glycine running buffer 10X
- Gram stain kit w/ grams iodine, crystal violet, saffron red
- Bromophenol blue
- ZnCl2 1M solution
- Kinetin 1mg/ml
- Alpha-Naphthaleneacetic acid 1mg/ml
Preapproved Organisms
The following organisms are permitted in the BUGSS facility as long as they are accompanied by documentation attesting to their identity and source. For organisms not on this white list specific written permission is required from the BUGSS safety officer (safety@bugssonline.org) before the organism can be allowed into the facility. This white list is a compilation taken from the WHO laboratory safety manual 3rd Edition, the NIH/CDC Biosafety in Biomedical research facilities and Biological Safety Principals and Practices 4th Edition.
Prokaryotic Microbes:
- Escherichia coli K-12 strains and derivatives:
- JM109
- DH5α
- HB101
- MC1066
- MM294
- Top 10 F’
- MG1655
- Escherichia coli B strains and derivatives:
- BL21 (DE3)
- Bacillus subtilus
- Thermus aquaticus
- Thermus thermophilus
- Geobacillus stearothermophilus
- Ralstonia metallidurans
Eukaryotic microbes:
- Saccharomyces cerevisiae S288C strains and derivatives:
- Pichia pastoris strains and derivatives
Mammalian cells:
- CHO (Chinese hamster ovary) cells and derivatives
Plant Cells:
- Arabidopsis thaliana and derivatives
Protozoa:
- Pyrocystis fusiformis