Molecular Biology Bootcamp: Building a Kill Switch in Bacteria

This class taught the fundamental techniques of molecular biology (PCR, restriction digest, ligation, and transformation) by cloning a regulated version of the holin gene which can then be activated to destroy bacteria.

Week 1

This week we performed PCR and gel electrophoresis and discussed what kill switches are.


Week 2

This week we performed Gibson Assembly and bacterial transformation and discussed bacterial toxin/antitoxin systems.