The Open Insulin Project is creating an open source (freely available) model for insulin production that centers sustainable, small-scale manufacturing and open source alternatives to production. We are developing organisms and protocols to produce rapid acting (lispro) and long acting (glargine) insulin. Additionally, we are working on developing open hardware equivalents to proprietary production equipment, are researching sustainable regulation pathways to bring our insulin to the public, and are developing plans for local, small-scale manufacturing pilots.