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PastSeminar

Unraveling the Mysteries of Long-COVID

We had a fantastic talk from Dr. Andrea (Andi) Levine, an Assistant Professor of Medicine in the division of Pulmonary & Critical Care Medicine at the University of Maryland School of Medicine. She discussed the current definition of Long-COVID syndrome, what we know about who gets it, why they do, and what we can do to try to both treat and prevent this syndrome.

Long-COVID comes by many names, but refers to symptoms that linger 1-3 months after initial infection, according to the CDC and WHO respectively. We are still very much in this pandemic; Dr. Levine highlighted that at-home testing likely led to the underreporting of overall cases. She highlighted that Long-COVID is its own pandemic and could lead to a mass deterioration event.

Long-COVID symptoms impact almost all organ systems and 80% of patients report at least one symptom that persists long-term. Roughly 50% of patients have ongoing symptoms after 1 month, 5 months, and up to 1 year. The majority of Long-COVID patients were female, obese or with underlying conditions, and around the 50-year age mark. The more symptoms you had earlier on made you more likely to experience Long-COVID symptoms.

The likelihood of a patient acquiring Long-COVID is related to their initial disease severity. A study in The Lancet found that even patients who were less sick initially still reported Long-COVID symptoms. However, that same study alluded that the sicker you were, the more likely you are to experience Long-COVID. The 2-dose vaccination series diminished the likelihood of experiencing persistent illness, but not entirely. Dr. Levine stressed the importance of getting boosters and mask-wearing. Getting vaccinated after COVID-19 infection and long-term symptoms have presented themselves may lead to remission of these symptoms as well. Different COVID variants triggered different persistence in whether patients experienced Long-COVID.

Dr. Levine discussed how the virus can persist in “viral reservoirs” within the body (i.e. the virus can be cleared in a nasal sample, but still present itself in the stool for weeks on end). She stressed again that all organ systems in patients reflect ongoing COVID-19 virus, meaning that no organ tissue is spared. The virus can cross the blood-brain barrier and continue to replicate. In these viral reservoirs (particularly the brain), the virus was seen to mutate from what it was at the time of initial infection. Significant areas of the brain maintained SARS-CoV-2 RNA and structural brain abnormalities could have led to neurological symptoms. COVID-19 infection also caused a prolonged inflammatory state in patients, leading to Long-COVID symptoms. Autoimmunity unmasking by COVID-19 infection is also a probable explanation.

Dr. Levine touched on the mental and social effects of Long-COVID. She discussed how many fear that their experiences are not real and all in their head. Long-COVID correlated with mental health diagnoses and the inability to work. Unfortunately, there are still a lot of unknowns on how inpatient and outpatient therapies impact Long-COVID. She highlighted we, as a society, are at a turning point and must begin to focus on persistent COVID symptoms in addition to the initial infections themselves.

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PastSeminar

Microbes in Space

 

The Microbes in Space seminar, co-sponsored by the Maryland Branch of the American Society for Microbiology, highlighted projects conducted in space relating to microbiology. Seminar attendees were joined by Dr. Jennifer Kerr of Notre Dame of Maryland University to present on some key studies happening in space and her own lab’s research.

Kerr first highlighted the many hazards spaceflight has on the human body. These dangers include radiation which damages DNA and lack of gravity which leads to mineral and bone loss. Therefore it is quite astonishing how certain microorganisms can withstand these obstacles, in particular tardigrades. Also known as water bears or moss piglets, tardigrades are tiny and cute invertebrates which prefer to live in water. They are well known for cryptobiosis, Latin for “hidden life”. Cryptobiosis is when there are no signs of metabolic activity, but the organism is still alive. In this state these animals only maintain 0.01% of normal metabolic activity which lets them handle extreme environments. Tardigrades shrivel up and go into what is known as the tun state during this dormancy period. There are also variations of cryptobiosis that tardigrades partake in. For instance, tardigrades in the tun state could survive 125 years without water (anhydrobiosis).

Tardigrade space research began in 2007 with the NASA Foton-M3 Mission studying radiation’s effects on these tiny critters. In a 2019 study, tardigrades in the tun state accidentally crash landed on the moon! Now, is it likely that there is a colony of tardigrades on the moon since there is no water on the moon and those tardigrades arrived in the tun state? Well, a 2021 study investigated tardigrade survival in such high-speed crashes. It was found that moss piglets could survive the impact, but not the shock pressure withstood, so it is unlikely that there is a tardigrade colony on the moon.

Aside from cute water bears, there are also more general microbiome studies happening in space. Specifically, these studies depict how contact surfaces around the International Space Station (ISS) have changed in response to the astronauts who come and go on the Space Station. The microbiomes of crewmembers may influence the microbial composition of ISS habitable surfaces. This is important in managing disease control and preventing contaminants from breaking certain hardware on the ISS. It was found that an astronaut’s microbiome contributes to roughly 55% of the environmental surface microbiome. These findings were not startling, yet it was importantly confirmed that the majority of these were safe and typical bacteria that already exists on the skin. However some were classified as opportunistic pathogens. Opportunistic pathogens have the potential to cause disease, but are unlikely to do so when kept in check by other bacteria or if the person’s immune system is properly functioning. Furthermore, this microbiome snapshot was maintained for a few weeks even after the particular astronaut had left. However this micro-diversity encountered turnover when a new astronaut arrived at the space station and was in constant interplay.

Not only did the microbiomes on ISS surfaces change, even the astronauts who lived there had notable shifts in their own microbial environments. In particular there were 347 bacterial species identified. This varied based on sample sites from the saliva, ears, skin, and nostrils. There were 12 top genera with the highest relative abundance identified across these astronaut samples. Mainly differences were seen in skin samples when astronauts were in flight to and from the Space Station. In the mouth, there were some key, but minimal changes. For instance, saliva had the largest change in composition of bacteria but relative abundance (which is the overall number of bacterial species) stayed the same. One potentially concerning finding with the saliva organisms was that some of them sampled displayed antimicrobial resistant gene markers. The reasoning is unknown, but further research is ongoing.

The NASA Biomedical Engineering for Exploration Space Tech (BEEST) lab is researching health care for exploration. Their goal is to train astronauts in non-invasive treatment of dental cavities. Seminar attendees were also shown a light-hearted video on how astronauts brush their teeth in space. Something as simple as brushing their teeth is even more important in space. There has never been an astronaut who is a dentist, so having preventative care and training is important. Astronauts are even taught techniques up to tooth extraction.

A healthy microbiome is known to be in eubiosis while an unbalanced one is in dysbiosis. For example, the reason that too much sugar leads to tooth decay is because bacteria in the mouth feed on this excess sugar. It causes them to grow more and create more acid. This excess acid leads to a pH shift in which creates a habitable environment for more hardy bacteria to in turn create more acid. This acid also destroys tooth enamel. This leads to cavities and even more body-based diseases. For instance, the dangerous bacteria from the mouth can move through blood to other bodily systems and clog arteries. Maintaining oral hygiene is of utmost priority in space.

This human-microbial research ties back to Dr. Jennifer Kerr’s research at Notre Dame. She is an oral microbiologist and studies teeth in space. Her work centers around Streptococcus mutans. Her lab hopes to help astronauts identify a cavity and use a handheld microwave device to kill the bacteria. Astonighly when this gadget is held to the mouth for only a minute, 99% of the S. mutans are killed. However, in the case of a cavity, demineralization still remains. In order to remineralize it, the astronaut’s body needs to be given the appropriate starting material and the pH has to come back to neutral. This research is still ongoing, but it could have profound impacts, not only on human health in space but even on Earth. According to the Global Burden of Disease Study “oral diseases affect close to 3.5 billion people worldwide” which is why this research and its findings will be so consequential to the world of dentistry and the science and medical communities as a whole.
 

Co-sponsored by the Maryland Branch of the American Society for Microbiology.

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PastSeminar

Digital Equity in Baltimore – Building a Shared Future Together

Last fall, with the promise of historic federal and state investments for community recovery, among other important initiatives, Baltimore City announced its Digital Equity Framework – a plan to permanently close its digital divide within the next eight years. Free public Wi-Fi in outdoor community gathering places has been announced as part of the plan, as has connecting our Rec Centers. The grand vision is a municipally owned fiber infrastructure serving all locations in the city. But this ambitious goal won’t be met by technology solutions alone.

Chris Ritzo presented to us about how the city is beginning this work and we discussed the Internet, Wi-Fi, and the power of human networks and community based solutions to combat inequities.

The seminar began with some background information and definitions. The Digital Divide is the gap between those with access to engage online and it disproportionately affects minorities and prevents equal technological access Digital Equity, on the other hand, differs from equality in that it acknowledges systemic barriers in place to hinder others. Lastly Digital Inclusion aims to involve all communities even those most disadvantaged. In order to achieve Digital Inclusion, there are five pillars which must be fulfilled. These include: affordable internet services, access to digital training, quality tech support, access to internet devices, and participation collaboration in the internet sphere. With online school and the overall shift online due to the COVID-19 pandemic, the Divide Divide problem has been further exacerbated and solutions for it become even more pressing.

Throughout the seminar there was active discussion from participants. A question was raised by one attendee on why internet within the City is worse than outside of it, even from the same provider. Another proposed this is because it is harder to lay underground lines in the City. Furthermore, Chris spoke to the disconnect between marketing and engineering sides at companies which also contributes to these problems. Another person highlighted the recent news of a sexual assault that occurred in the Facebook Metaverse. The victim had received responses along the lines of “if you don’t like it then don’t join”. Chris mentioned how this current event ties back to the definition of Digital Inclusion and how moderating community norms is important in addition to creating these novel tools. He also suggested a book, Behind the Screen by Sarah Roberts, identifying the problematic issues of social media. Around the ongoing purchase of Twitter by Elon Musk, Ritzo highlighted Twitter and other social media networks’ claims to support free speech, but how they can never be truly utopian since the judgment lies within the corporation with an end goal of data mining and advertising to its users.

The seminar moved on to discuss technical aspects of getting access to the Internet. For instance, a router helps us connect wired/wirelessly to a laptop onto the Internet. This router also protects us and our data to some extent via firewall. First Mile is the idea of democratizing internet setup. In this concept, individuals can also set up Wi-Fi services, not just big corporations. The seminar also touched upon running speed tests. Chris said speed is important, but not the only thing that matters. It was recommended to survey nearby Wi-Fi channels, buy an extender, and understand the channel overlaps of your current network and where it should be. There were also online sites provided to see how crowded the channels are in your neighborhood. In the same vein, conduct basic latency tests, in particular latency under load also known as bufferbloat, to determine where your internet stands as it buffers in the case there is too much data.

Overall, data mechanics surrounding internet accessibility maintains a key driver in creating a community based solution to this problem. Science and technology will benefit greatly when there is contribution and inclusion in which there is equitable and diverse representation across the Internet.

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SWEET Science: Responsible Bioengineering for Amateurs and Educators

A plant geneticist’s discussion on alternative methods of bioengineering.

Sebastion Cocioba discussed using sugars as a means of selection in molecular cloning and plant genetic engineering, removing antibiotics and herbicides from the equation entirely.

Sebastian Cocioba is a plant biotechnology researcher with a focus on the production of commercially and industrially valuable plant species. He is an owner of New York Botanics, LLC, a plant biotech R&D laboratory with a specialization in orchid micropropagation, a founder of Binomica Labs (https://binomicalabs.org/), and a leader in the open science movement. Follow him at: https://twitter.com/atinygreencell

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Counting Shrimp with Sonar

Ever wonder where shrimp come from? Shrimp farming is harder than you might think! Agriculture and aquaculture farmers need to understand how many plants and animals they are growing on land or in the water to make decisions on their farm. For aqua-farmers counting shrimp is a major challenge because their animals are grown in murky water and the farmers are blindfolded to how many shrimp they have. Minnowtech aims to help the farmers by counting their shrimp using sonar and the math behind their behavior, providing aqua-farmers with the information they need to manage their farm efficiently. In this seminar, Suzan Shahrestani of Minnowtech tells us how shrimp and other seafood get to your dinner plate, and how Minnowtech is striving to make that process easier for farmers.

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Computer Aided Drug Design: Taking an Idea to the Clinic

Computer-aided drug design (CADD) methods have made a significant impact on the discovery and development of new drugs for treating disease. Our speakers, Alexander MacKerell and Paul Shapiro, Professors of Pharmaceutical Sciences at the University of Maryland School of Medicine, provided us with an an overview of CADD approaches and their applications in designing new drugs that target enzymes involved in cancer and inflammation.

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3D Bioprinting Made Easy

You may have heard of bioprinting, where cells and biomaterials can be printed to create custom organs and tissues. But did you know that bioprinting can be achieved through the same techniques used to get graphics printed on a t-shirt? This talk on bioprinting discusses unique strategies to bioprint at home!

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Healthy Harbor and Mr. Trash Wheel

What is a trash wheel? What role do they play in protecting Baltimore’s harbor and wildlife from pollution? Adam Lindquist, the director of Healthy Harbor, answered all of our questions about our trash-intercepting, googly-eyed neighbors and the rest of Healthy Harbor’s initiatives!

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Rethinking Meat to Feed 10 billion

Growing meat directly from plants, microbes, and animal cells will allow us to build a food system that is better for human, animal, and planetary health. However, catalyzing this paradigm shift is a vast, multidisciplinary effort that requires scientists and engineers from disciplines ranging from tissue engineering and synthetic biology to computational science and chemical engineering. Join The Good Food Institute’s Amy Huang to explore the state of alternative protein research with a focus on illuminating the research white spaces that need to be filled if we’re to power a transition away from industrialized animal agriculture.

Bio of the speaker: Amy Huang is the University Innovation Manager at The Good Food Institute. At GFI, Amy works with students and faculty around the world to turn universities into hubs for alternative protein research and education. In addition to working with scientists and engineers to address priority white spaces in plant-based and cultivated meat technologies, she focuses on bringing diverse stakeholders together through the formation of student groups, global scientific communities, and interdisciplinary research centers. Amy graduated from Harvard University with an AB in economics and global health.

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Making plastics from methane

Mango Materials is a San Francisco-based company that manufactures biodegradable materials using bacteria that feed on waste biogas (methane). The company’s end product is a naturally occurring polyhydroxyalkanoate (PHA) polymer that can biodegrade in many different environments. Since 2012, Mango has developed PHA that can be used to create textile fibers as a polyester replacement and that can be used to create injection molded packaging for the cosmetics industry.

This talk by Dr. Anne Schauer-Gimenez, Vice President of Customer Engagement and co-founder, discussed the journey from methane to end-product application, end-of-life biodegradability, and next steps for the company as it transitions to commercialization.